Search results for "Intrinsically disordered region"

showing 5 items of 5 documents

Large-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications

2021

The highly infectious disease COVID-19 caused by the Betacoronavirus SARS-CoV-2 poses a severe threat to humanity and demands the redirection of scientific efforts and criteria to organized research projects. The international COVID19-NMR consortium seeks to provide such new approaches by gathering scientific expertise worldwide. In particular, making available viral proteins and RNAs will pave the way to understanding the SARS-CoV-2 molecular components in detail. The research in COVID19-NMR and the resources provided through the consortium are fully disclosed to accelerate access and exploitation. NMR investigations of the viral molecular components are designated to provide the essential…

Life sciences; biologySARS-COV-2; COVID-19; protein production; structural biology NMR[SDV.BIO]Life Sciences [q-bio]/BiotechnologyBiochemistry Genetics and Molecular Biology (miscellaneous)BiochemistryAccessory proteinsNMR spectroscopyddc:570[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN]Molecular Biosciencesddc:610Nonstructural proteinsMolecular BiologyOriginal Research[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry Molecular Biology/Structural Biology [q-bio.BM]SARS-CoV-2Intrinsically disordered regionnonstructural proteinsCOVID-19structural proteinsCell-free protein synthesisintrinsically disordered regioncell-free protein synthesisaccessory proteins[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/VirologyStructural proteins
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The Role of Low Complexity Regions in Protein Interaction Modes: An Illustration in Huntingtin

2021

Low complexity regions (LCRs) are very frequent in protein sequences, generally having a lower propensity to form structured domains and tending to be much less evolutionarily conserved than globular domains. Their higher abundance in eukaryotes and in species with more cellular types agrees with a growing number of reports on their function in protein interactions regulated by post-translational modifications. LCRs facilitate the increase of regulatory and network complexity required with the emergence of organisms with more complex tissue distribution and development. Although the low conservation and structural flexibility of LCRs complicate their study, evolutionary studies of proteins …

Protein Conformation alpha-Helical0301 basic medicineNetwork complexityHuntingtinintrinsically disordered regionsAmino Acid MotifsComputational biologyBiologyprotein interactionsArticlecompositionally biased regionsCatalysisProtein–protein interactionlcsh:ChemistryEvolution MolecularInorganic ChemistryLow complexity03 medical and health sciencesProtein DomainsProtein Interaction MappingAnimalsHumansp300-CBP Transcription FactorsAmino Acid SequenceProtein Interaction MapsHuntingtinTissue distributionPhysical and Theoretical Chemistrylcsh:QH301-705.5Molecular BiologySpectroscopyHuntingtin Protein030102 biochemistry & molecular biologyOrganic ChemistryNuclear Proteinsp120 GTPase Activating ProteinGeneral MedicineMultiple modesSynapsinslow complexity regionsComputer Science ApplicationshomorepeatsMicroscopy Electron030104 developmental biologylcsh:Biology (General)lcsh:QD1-999Sequence AlignmentFunction (biology)Protein BindingInternational Journal of Molecular Sciences
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Editorial: The Role of Protein Post-Translational Modifications in Protein-RNA Interactions and RNP Assemblies

2022

RNP granulesEditorialQH301-705.5Molecular BiosciencesRNA-protein interactionsPTMs (post-translational modifications)phase separationBiology (General)Biochemistry Genetics and Molecular Biology (miscellaneous)Molecular BiologyBiochemistryintrinsically disordered region (IDR)Frontiers in Molecular Biosciences
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Evolutionary Study of Disorder in Protein Sequences

2020

Intrinsically disordered proteins (IDPs) contain regions lacking intrinsic globular structure (intrinsically disordered regions, IDRs). IDPs are present across the tree of life, with great variability of IDR type and frequency even between closely related taxa. To investigate the function of IDRs, we evaluated and compared the distribution of disorder content in 10,695 reference proteomes, confirming its high variability and finding certain correlation along the Euteleostomi (bony vertebrates) lineage to number of cell types. We used the comparison of orthologs to study the function of disorder related to increase in cell types, observing that multiple interacting subunits of protein comple…

intrinsically disordered regionsortholog comparisonLineage (evolution)High variabilitylcsh:QR1-502comparative genomicsBiologyIntrinsically disordered proteinsBiochemistryArticlelcsh:MicrobiologyEvolution Molecular03 medical and health sciencesSequence Analysis ProteinAnimalsDatabases ProteinMolecular Biology030304 developmental biologyComparative genomics0303 health sciences030302 biochemistry & molecular biologyEvolutionary biologyVertebratesProteomeintrinsically disordered proteinsFunction (biology)Biomolecules
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RNA-Binding Proteins as Epigenetic Regulators of Brain Functions and Their Involvement in Neurodegeneration.

2022

A central aspect of nervous system development and function is the post-transcriptional regulation of mRNA fate, which implies time- and site-dependent translation, in response to cues originating from cell-to-cell crosstalk. Such events are fundamental for the establishment of brain cell asymmetry, as well as of long-lasting modifications of synapses (long-term potentiation: LTP), responsible for learning, memory, and higher cognitive functions. Post-transcriptional regulation is in turn dependent on RNA-binding proteins that, by recognizing and binding brief RNA sequences, base modifications, or secondary/tertiary structures, are able to control maturation, localization, stability, and tr…

learningsynaptic plasticityOrganic ChemistryneurodegenerationRNA-Binding ProteinsBrainGeneral MedicineCatalysisComputer Science ApplicationsmemoryInorganic ChemistryIntrinsically Disordered ProteinsGene Expression RegulationSettore BIO/10 - BiochimicaRNA-binding proteins (RBPs)Settore MED/26 - NeurologiaNervous System Physiological PhenomenaRNA Messengerpost-transcriptional regulation of gene expressionSettore BIO/06 - Anatomia Comparata E CitologiaPhysical and Theoretical ChemistryEVsMolecular Biologyintrinsically disordered regions (IDRs)SpectroscopyInternational journal of molecular sciences
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